human skin melanoma cell line sh 4  (ATCC)

Journal: bioRxiv

Article Title: Functional and sensitivity profiling of the KIT Mutation Landscape in Melanoma

doi: 10.64898/2026.02.18.706482

Figure Lengend Snippet: KIT mutations shows differential responses towards common KIT inhibitors in vitro. (A) Western Blot analysis revealed changes in cell growth and proliferation pathways upon expression of KIT mutants in MeWO melanoma cells. (B) Upper panel: Monitoring relative growth of melanoma cell spheroids expressing KIT mutants using high-content microscopy. Lower panel: Representative image depicting spheroid size after 8 days of growth in 3D environment. (C) Western Blot demonstrating the expression of KIT in Ba/F3 cells. (D) Assessment of relative growth of Ba/F3 cells expressing different KIT mutants following withdrawal of IL-3 from the culture medium, measured using cell-titre glo assay. (E) Heatmap shows the relative IC50 of Ba/F3 cell with KIT mutants against KIT inhibitors. (F) Violin plot illustrating the IC50 values of MeWo cells expressing KIT mutants when treated with various KIT inhibitors. Results were obtained from three independent experiments. The horizontal line represents the mean, and statistical analysis was performed using one-way ANOVA.

Article Snippet: The IL-3-dependent murine pro-B cell line Ba/F3 and the human melanoma cell line MeWo (ATCC) were used for functional characterization.

Techniques: In Vitro, Western Blot, Expressing, Microscopy, Glo Assay

Journal: Molecules

Article Title: Anticancer Activity of Microbial Biosurfactants Amphisin and Viscosinamide Against Melanoma Cells

doi: 10.3390/molecules31040668

Figure Lengend Snippet: Cytotoxicity of amphisin toward A375, B16 4A5 and NHDF cells after 24 h ( a ) and 48 h ( b ) of incubation. Results are presented as mean ± SD from three independent experiments. Statistical analysis was performed using Student’s t -test. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Human melanoma A375 (ATCC CRL-1619; Manassas, VA, USA) and mouse melanoma B16 4A5 cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Sigma-Aldrich, St. Louis, MO, USA) enriched with 10% fetal bovine serum (FBS, Gibco Life Technologies, Carlsbad, CA, USA) 1% L-glutamine (Sigma-Aldrich, St. Louis, MO, USA), and 1% antibiotics solution containing penicillin (10 U/mL) and streptomycin (10 μg/mL) (Sigma-Aldrich, St. Louis, MO, USA).

Techniques: Incubation

Journal: Molecules

Article Title: Anticancer Activity of Microbial Biosurfactants Amphisin and Viscosinamide Against Melanoma Cells

doi: 10.3390/molecules31040668

Figure Lengend Snippet: IC 50 values for A375 ( a ) B16 4A5 ( b ) and NHDF ( c ) cells after 24 h of incubation with amphisin.

Article Snippet: Human melanoma A375 (ATCC CRL-1619; Manassas, VA, USA) and mouse melanoma B16 4A5 cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Sigma-Aldrich, St. Louis, MO, USA) enriched with 10% fetal bovine serum (FBS, Gibco Life Technologies, Carlsbad, CA, USA) 1% L-glutamine (Sigma-Aldrich, St. Louis, MO, USA), and 1% antibiotics solution containing penicillin (10 U/mL) and streptomycin (10 μg/mL) (Sigma-Aldrich, St. Louis, MO, USA).

Techniques: Incubation

Journal: Molecules

Article Title: Anticancer Activity of Microbial Biosurfactants Amphisin and Viscosinamide Against Melanoma Cells

doi: 10.3390/molecules31040668

Figure Lengend Snippet: Cytotoxicity of viscosinamide toward A375, B16 4A5 and NHDF cells after 24 h ( a ) and 48 h ( b ) of incubation. Results are presented as mean ± SD from three independent experiments. Statistical analysis was performed using Student’s t -test. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Human melanoma A375 (ATCC CRL-1619; Manassas, VA, USA) and mouse melanoma B16 4A5 cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Sigma-Aldrich, St. Louis, MO, USA) enriched with 10% fetal bovine serum (FBS, Gibco Life Technologies, Carlsbad, CA, USA) 1% L-glutamine (Sigma-Aldrich, St. Louis, MO, USA), and 1% antibiotics solution containing penicillin (10 U/mL) and streptomycin (10 μg/mL) (Sigma-Aldrich, St. Louis, MO, USA).

Techniques: Incubation

Journal: Molecules

Article Title: Anticancer Activity of Microbial Biosurfactants Amphisin and Viscosinamide Against Melanoma Cells

doi: 10.3390/molecules31040668

Figure Lengend Snippet: IC 50 values for A375 ( a ), B16 4A5 ( b ) and NHDF ( c ) cell after 24 h of incubation with viscosinamide.

Article Snippet: Human melanoma A375 (ATCC CRL-1619; Manassas, VA, USA) and mouse melanoma B16 4A5 cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Sigma-Aldrich, St. Louis, MO, USA) enriched with 10% fetal bovine serum (FBS, Gibco Life Technologies, Carlsbad, CA, USA) 1% L-glutamine (Sigma-Aldrich, St. Louis, MO, USA), and 1% antibiotics solution containing penicillin (10 U/mL) and streptomycin (10 μg/mL) (Sigma-Aldrich, St. Louis, MO, USA).

Techniques: Incubation

Journal: Molecules

Article Title: Anticancer Activity of Microbial Biosurfactants Amphisin and Viscosinamide Against Melanoma Cells

doi: 10.3390/molecules31040668

Figure Lengend Snippet: Effect of amphisin ( a ) and viscosinamide ( b ) on LDH release in A375, B16 4A5 and NHDF cells after 24 h of incubation. Results are presented as mean ± SD ( n = 3). Statistical analysis was performed using Student’s t -test. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Human melanoma A375 (ATCC CRL-1619; Manassas, VA, USA) and mouse melanoma B16 4A5 cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Sigma-Aldrich, St. Louis, MO, USA) enriched with 10% fetal bovine serum (FBS, Gibco Life Technologies, Carlsbad, CA, USA) 1% L-glutamine (Sigma-Aldrich, St. Louis, MO, USA), and 1% antibiotics solution containing penicillin (10 U/mL) and streptomycin (10 μg/mL) (Sigma-Aldrich, St. Louis, MO, USA).

Techniques: Incubation

Journal: Molecules

Article Title: Anticancer Activity of Microbial Biosurfactants Amphisin and Viscosinamide Against Melanoma Cells

doi: 10.3390/molecules31040668

Figure Lengend Snippet: Phosphatidylserine translocation induced by amphisin, viscosinamide and staurosporine in A375 and B16 4A5 cells. Representative fluorescence microscopy images acquired with ZEN software version 3.0 (Carl Zeiss, Jena, Germany). Scale bar: 200 µm.

Article Snippet: Human melanoma A375 (ATCC CRL-1619; Manassas, VA, USA) and mouse melanoma B16 4A5 cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Sigma-Aldrich, St. Louis, MO, USA) enriched with 10% fetal bovine serum (FBS, Gibco Life Technologies, Carlsbad, CA, USA) 1% L-glutamine (Sigma-Aldrich, St. Louis, MO, USA), and 1% antibiotics solution containing penicillin (10 U/mL) and streptomycin (10 μg/mL) (Sigma-Aldrich, St. Louis, MO, USA).

Techniques: Translocation Assay, Fluorescence, Microscopy, Software

Journal: Molecules

Article Title: Anticancer Activity of Microbial Biosurfactants Amphisin and Viscosinamide Against Melanoma Cells

doi: 10.3390/molecules31040668

Figure Lengend Snippet: Nuclear morphology of control A375 and B16 4A5 cells and cells treated with amphisin, viscosinamide and staurosporine, assessed by fluorescence microscopy. Images were acquired using ZEN software version 3.0 (Carl Zeiss, Jena, Germany). Scale bar: 200 µm.

Article Snippet: Human melanoma A375 (ATCC CRL-1619; Manassas, VA, USA) and mouse melanoma B16 4A5 cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Sigma-Aldrich, St. Louis, MO, USA) enriched with 10% fetal bovine serum (FBS, Gibco Life Technologies, Carlsbad, CA, USA) 1% L-glutamine (Sigma-Aldrich, St. Louis, MO, USA), and 1% antibiotics solution containing penicillin (10 U/mL) and streptomycin (10 μg/mL) (Sigma-Aldrich, St. Louis, MO, USA).

Techniques: Control, Fluorescence, Microscopy, Software

Journal: Molecules

Article Title: Anticancer Activity of Microbial Biosurfactants Amphisin and Viscosinamide Against Melanoma Cells

doi: 10.3390/molecules31040668

Figure Lengend Snippet: Expression of pro- and anti-apoptotic genes in A375 ( a ) and B16 4A5 ( b ) cells treated with amphisin. Results are presented as mean ± SD ( n = 3). Statistical analysis was performed using Student’s t -test. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Human melanoma A375 (ATCC CRL-1619; Manassas, VA, USA) and mouse melanoma B16 4A5 cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Sigma-Aldrich, St. Louis, MO, USA) enriched with 10% fetal bovine serum (FBS, Gibco Life Technologies, Carlsbad, CA, USA) 1% L-glutamine (Sigma-Aldrich, St. Louis, MO, USA), and 1% antibiotics solution containing penicillin (10 U/mL) and streptomycin (10 μg/mL) (Sigma-Aldrich, St. Louis, MO, USA).

Techniques: Expressing

Journal: Molecules

Article Title: Anticancer Activity of Microbial Biosurfactants Amphisin and Viscosinamide Against Melanoma Cells

doi: 10.3390/molecules31040668

Figure Lengend Snippet: Expression of pro- and anti-apoptotic genes in A375 ( a ) and B16 4A5 ( b ) cells treated with viscosinamide. Results are presented as mean ± SD ( n = 3). Statistical analysis was performed using Student’s t -test. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Human melanoma A375 (ATCC CRL-1619; Manassas, VA, USA) and mouse melanoma B16 4A5 cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Sigma-Aldrich, St. Louis, MO, USA) enriched with 10% fetal bovine serum (FBS, Gibco Life Technologies, Carlsbad, CA, USA) 1% L-glutamine (Sigma-Aldrich, St. Louis, MO, USA), and 1% antibiotics solution containing penicillin (10 U/mL) and streptomycin (10 μg/mL) (Sigma-Aldrich, St. Louis, MO, USA).

Techniques: Expressing

Journal: Molecules

Article Title: Anticancer Activity of Microbial Biosurfactants Amphisin and Viscosinamide Against Melanoma Cells

doi: 10.3390/molecules31040668

Figure Lengend Snippet: Representative images of the migration analysis of A375 cells treated with amphisin and viscosinamide compared with untreated control cells at 0 h, 24 h, and 48 h of incubation. Scale bar: 200 µm.

Article Snippet: Human melanoma A375 (ATCC CRL-1619; Manassas, VA, USA) and mouse melanoma B16 4A5 cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Sigma-Aldrich, St. Louis, MO, USA) enriched with 10% fetal bovine serum (FBS, Gibco Life Technologies, Carlsbad, CA, USA) 1% L-glutamine (Sigma-Aldrich, St. Louis, MO, USA), and 1% antibiotics solution containing penicillin (10 U/mL) and streptomycin (10 μg/mL) (Sigma-Aldrich, St. Louis, MO, USA).

Techniques: Migration, Control, Incubation